Dynamic Protease Activation on a Multimeric Synthetic Protein Scaffold via Adaptable DNA‐Based Recruitment Domains

Hexameric hemoprotein (HTHP) is employed as a scaffold protein for the supramolecular assembly and activation of apoptotic signalling enzyme caspase-9, using short DNA elements modular recruitment domains. Caspase-9 on HTHP platform due to enhanced proximity followed by combinatorial inhibition at high concentrations. The domains allow reversible switching caspase-9 activity state modulatory strands. Tuning domain affinity allows generating kinetically trapped active complexes, well dynamic repositioning caspases over populations monovalent sink platforms. conceptual combination highly structured multivalent with provides emergent biomimicry properties advanced levels control assembly.